7CYB

Saimiri boliviensis boliviensis galectin-13 with glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	33.407, 58.913, 40.701
Unit cell angles	90.00, 113.34, 90.00

Refinement procedure

Resolution	19.820 - 1.600
R-factor	0.1571
Rwork	0.154
R-free	0.18410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kjx
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.820	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.034	0.099
Number of reflections	18965	929
<I/σ(I)>	19.5
Completeness [%]	98.9
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG