7CY6
Crystal Structure of CMD1 in complex with 5mC-DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.173, 127.055, 64.217 |
Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.100 |
R-factor | 0.1846 |
Rwork | 0.182 |
R-free | 0.22730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cy4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.179 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.069 | 0.840 |
Number of reflections | 62383 | 3199 |
<I/σ(I)> | 18.6 | 2.2 |
Completeness [%] | 96.6 | |
Redundancy | 6.7 | |
CC(1/2) | 1.000 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 15% (v/v) 2-propanol, 0.1 M sodium citrate, pH 5.0, and 10% (w/v) PEG 10000 |