7CY2
The open conformation of MSMEG_1954 from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97876 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.006, 74.006, 167.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.940 - 2.750 |
| R-factor | 0.2431 |
| Rwork | 0.241 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yjz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.115 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.810 |
| High resolution limit [Å] | 2.750 | 6.780 | 2.750 |
| Rmerge | 0.122 | 0.058 | 1.758 |
| Rmeas | 0.125 | 0.060 | 1.799 |
| Rpim | 0.025 | 0.013 | 0.375 |
| Total number of observations | 312083 | ||
| Number of reflections | 12763 | 975 | 811 |
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 24.5 | 22.6 | 21.1 |
| CC(1/2) | 0.996 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium sulfate, 0.1M Tris (pH8.5), 25% w/v PEG 3350 |
