7CY1

Crystal Structure of MglC from Myxococcus xanthus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98000
Spacegroup name	P 65 2 2
Unit cell lengths	96.910, 96.910, 58.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.980 - 2.190
R-factor	0.1904
Rwork	0.188
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ct3
RMSD bond length	0.007
RMSD bond angle	1.462
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.980	27.980	2.260
High resolution limit [Å]	2.190	9.040	2.190
Rmerge	0.075	0.025	0.959
Total number of observations	154682	2076	11159
Number of reflections	8534	151	708
<I/σ(I)>	26.1	69.5	3.9
Completeness [%]	98.8	95.2	97.2
Redundancy	18.1	13.7	15.8
CC(1/2)	1.000	1.000	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	2.8 M Sodium Acetate Trihydrate pH 7.0