7CXO
Crystal structure of Arabinose isomerase from hybrid AI10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2020-07-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 148.476, 258.575, 165.734 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.890 - 3.200 |
R-factor | 0.1802 |
Rwork | 0.176 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ajt |
RMSD bond length | 0.006 |
RMSD bond angle | 1.304 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.187 | 0.063 | 0.975 |
Rmeas | 0.197 | 0.067 | 1.031 |
Rpim | 0.062 | 0.021 | 0.328 |
Number of reflections | 54358 | 2845 | 2690 |
<I/σ(I)> | 3.1 | ||
Completeness [%] | 99.8 | 98 | 99.9 |
Redundancy | 9.5 | 9.3 | 9.2 |
CC(1/2) | 0.999 | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% (v/v) Polyethylene glycol 400, 100mM Sodium cacodylate/ Hydrochloric acid pH 6.5, 200mM Lithium sulfate |