7CX5

Crystal structure of the DNA-binding domain of Bacillus subtilis CssR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	29.410, 52.740, 58.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.320 - 1.070
R-factor	0.1949
Rwork	0.194
R-free	0.20410
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.320	1.080
High resolution limit [Å]	1.070	1.070
Rmerge	0.075	0.655
Number of reflections	41035	2021
<I/σ(I)>	12.6
Completeness [%]	98.5
Redundancy	5.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8.5	293	2 M Lithium sulfate, 2% (v/v) PEG400, and 0.1 M Tris/HCl pH 8.5