7CX1

Crystal structure of a tyrosine decarboxylase from Enterococcus faecalis in complex with the cofactor PLP and inhibitor methyl-tyrosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-23
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 61 2 2
Unit cell lengths	131.490, 131.490, 385.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.850 - 2.540
R-factor	0.2121
Rwork	0.210
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hsi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.850
High resolution limit [Å]	2.540	5.900	2.750
Rmerge	0.151	0.050	1.094
Rmeas	0.153	0.051	1.114
Rpim	0.029	0.010	0.209
Number of reflections	65687	5840	5267
<I/σ(I)>	3.9
Completeness [%]	100.0	99.9	100
Redundancy	29	25	27.9
CC(1/2)		0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15% PEG 1000, 0.2 M Mgcl2 and 0.1 M sodium citrate tribasic dehydrate (pH 6.5)