7CWX

Crystal structure of a tyrosine decarboxylase from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-27
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9998
Spacegroup name	P 61 2 2
Unit cell lengths	132.338, 132.338, 391.483
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.960 - 2.150
R-factor	0.194
Rwork	0.192
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hsi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.230
High resolution limit [Å]	2.150	4.620	2.150
Rmerge	0.102	0.046
Rmeas	0.104	0.047
Rpim	0.023	0.010	0.261
Total number of observations	2388870
Number of reflections	110354	11795	10794
<I/σ(I)>	5.8
Completeness [%]	99.6	99.7	99.2
Redundancy	21.6	21.1	16.9
CC(1/2)		1.000	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	15% PEG 1000, 0.2 M Mgcl2, 0.1 M sodium citrate tribasic dehydrate (pH 6.5)