7CWP
Root induced Secreted protein Tsp1 from Biocontrol fungi Trichoderma virens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.200, 66.700, 168.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.994 - 2.100 |
R-factor | 0.1785 |
Rwork | 0.176 |
R-free | 0.22550 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.765 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.890 | 42.890 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.115 | 0.047 | 0.484 |
Rmeas | 0.129 | 0.054 | 0.541 |
Rpim | 0.055 | 0.025 | 0.234 |
Total number of observations | 131390 | 1711 | 10730 |
Number of reflections | 29074 | 404 | 2413 |
<I/σ(I)> | 9.4 | 19.6 | 3.1 |
Completeness [%] | 95.8 | 87.4 | 97.1 |
Redundancy | 4.5 | 4.2 | 4.4 |
CC(1/2) | 0.994 | 0.996 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.9 M sodium malonate pH 6.5, 5 mM glucose, 5% glycerol |