7CWK

Structure of a triple-helix region of human collagen type I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-08-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	90.354, 19.350, 37.110
Unit cell angles	90.00, 110.59, 90.00

Refinement procedure

Resolution	23.147 - 1.539
R-factor	0.2099
Rwork	0.208
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d3h
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.147	1.594
High resolution limit [Å]	1.539	1.539
Number of reflections	7870	439
<I/σ(I)>	13.86
Completeness [%]	85.0
Redundancy	7.3
CC(1/2)	0.997	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Potassium Na tartrate tetrahydrate, 20% PEG3350