7CVQ
crystal structure of Arabidopsis CO CCT domain in complex with NF-YB2/YC3 and FT CORE1 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.244, 65.273, 138.284 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 35.181 - 3.300 |
| R-factor | 0.2425 |
| Rwork | 0.241 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cvo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.148 | 0.811 |
| Number of reflections | 28108 | 2727 |
| <I/σ(I)> | 7.7 | 1 |
| Completeness [%] | 99.2 | 97.4 |
| Redundancy | 4.4 | 4.1 |
| CC(1/2) | 0.996 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1 M CHES, pH 9.5 and 40% PEG 400 |
