7CVA
RNA methyltransferase METTL4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.812, 94.323, 95.769 |
| Unit cell angles | 90.00, 101.75, 90.00 |
Refinement procedure
| Resolution | 29.668 - 2.500 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (7.0.076) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.132 | 0.101 | 0.413 |
| Rmeas | 0.148 | 0.112 | 0.491 |
| Rpim | 0.065 | 0.048 | 0.258 |
| Number of reflections | 65279 | 3711 | 3421 |
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 98.2 | 98.8 | 93.7 |
| Redundancy | 4.8 | 5.4 | 3.3 |
| CC(1/2) | 0.987 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M tacsimate pH 5.5 and 8% PEG3350 |
