7CV9
RNA methyltransferase METTL4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.603, 93.545, 96.612 |
Unit cell angles | 90.00, 104.78, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.455 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.25410 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.974 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.030 | 30.000 | 2.540 |
High resolution limit [Å] | 2.450 | 5.270 | 2.450 |
Rmerge | 0.104 | 0.074 | 0.392 |
Rmeas | 0.114 | 0.080 | 0.442 |
Rpim | 0.046 | 0.031 | 0.198 |
Number of reflections | 39010 | 4075 | 3536 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 97.6 | 99.9 | 88.6 |
Redundancy | 5.3 | 6.5 | 4 |
CC(1/2) | 0.991 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1600 mM Magnesium sulfate 100 mM MES/ Sodium hydroxide pH 6.5 |