7CV8

RNA methyltransferase METTL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	52.092, 84.404, 93.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.370
R-factor	0.2009
Rwork	0.198
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.438
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.960	30.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.180	0.139	0.608
Rmeas	0.188	0.146	0.640
Rpim	0.054	0.044	0.195
Number of reflections	17743	1940	1596
<I/σ(I)>	4.7
Completeness [%]	98.8	99.9	91.5
Redundancy	11.8	11.5	8.6
CC(1/2)		0.992	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1M Lithium chloride 0.1M HEPES pH 7.0 10% PEG6000