7CV1
Structure of human tRNAHis guanylyltransferase (Thg1) in the presence of human mitochondrial tRNAHis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-17 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 155.878, 155.878, 201.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.462 - 4.000 |
R-factor | 0.2436 |
Rwork | 0.242 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ote |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.462 | 49.460 | 4.280 |
High resolution limit [Å] | 4.000 | 11.310 | 4.000 |
Rmerge | 1.011 | 0.096 | 7.893 |
Rmeas | 1.036 | 0.099 | 8.085 |
Rpim | 0.226 | 0.022 | 1.742 |
Total number of observations | 22714 | 93005 | |
Number of reflections | 24468 | 1173 | 4363 |
<I/σ(I)> | 5.9 | 21.5 | 1.2 |
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 20.4 | 19.4 | 21.3 |
CC(1/2) | 0.989 | 0.997 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl pH 7.0, 10% (w/v) PEG6000, 5% (w/v) Tacsimate pH7.0 |