7CUS

Crystal structure of the sensor domain of VbrK from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	29.686, 69.875, 106.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 1.650
R-factor	0.1833
Rwork	0.182
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r7q
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.116	0.435
Number of reflections	27602	1334
<I/σ(I)>	22	3.2
Completeness [%]	99.8	99.9
Redundancy	6.4	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350, di-ammonium hydrogen phosphate