7CUS
Crystal structure of the sensor domain of VbrK from Vibrio parahaemolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.686, 69.875, 106.498 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.210 - 1.650 |
R-factor | 0.1833 |
Rwork | 0.182 |
R-free | 0.20860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r7q |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.116 | 0.435 |
Number of reflections | 27602 | 1334 |
<I/σ(I)> | 22 | 3.2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.4 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 3350, di-ammonium hydrogen phosphate |