7CUP
Structure of 2,5-dihydroxypridine Dioxygenase from Pseudomonas putida KT2440
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 125.961, 143.750, 118.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.410 - 2.000 |
| R-factor | 0.1718 |
| Rwork | 0.171 |
| R-free | 0.21340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cnt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.625 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 142933 | 6863 |
| <I/σ(I)> | 25 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 | |
| CC(1/2) | 1.000 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350 0.2 M Succinic acid pH 7.0 |
