7CUJ
Crystal structure of fission yeast Ccq1 and Tpz1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.300, 71.752, 95.180 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 38.870 - 2.400 |
| R-factor | 0.1995 |
| Rwork | 0.197 |
| R-free | 0.25510 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.831 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.058 | 0.032 | 0.593 |
| Rmeas | 0.066 | 0.036 | 0.676 |
| Rpim | 0.030 | 0.016 | 0.318 |
| Total number of observations | 131622 | ||
| Number of reflections | 29579 | 3077 | 2746 |
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 98.9 | 99.8 | 92 |
| Redundancy | 4.4 | 4.5 | 4 |
| CC(1/2) | 0.998 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.8 | 277 | 15% PEG4000, 200mM potassium chloride, 50mM magnesium chloride and 50mM Tris-HCl |
| 1 | EVAPORATION | 7.8 | 277 | 15% PEG4000, 200mM potassium chloride, 50mM magnesium chloride and 50mM Tris-HCl |
