7CUH
Crystal structure of fission yeast Pot1 and ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.212, 65.903, 92.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.440 - 3.000 |
R-factor | 0.2689 |
Rwork | 0.267 |
R-free | 0.29760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qzg 4hik |
RMSD bond length | 0.005 |
RMSD bond angle | 1.282 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.137 | 0.076 | 0.494 |
Rmeas | 0.149 | 0.083 | 0.545 |
Rpim | 0.059 | 0.034 | 0.225 |
Total number of observations | 49420 | ||
Number of reflections | 8002 | 883 | 737 |
<I/σ(I)> | 4.5 | ||
Completeness [%] | 99.3 | 99.8 | 95.2 |
Redundancy | 6.2 | 5.9 | 5.5 |
CC(1/2) | 0.994 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 277 | 20% PEG3350, 100 mM ammonium sulfate and 100 mM Bis-Tris |
1 | EVAPORATION | 5.5 | 277 | 20% PEG3350, 100 mM ammonium sulfate and 100 mM Bis-Tris |