7CUC
Crystal Structure of Urate Oxidase from Bacillus sp. TB-90 in the absence from Chloride Anion at 1.44 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-17 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 71.618, 133.836, 145.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.870 - 1.440 |
R-factor | 0.1771 |
Rwork | 0.175 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wlv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.064 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmerge | 0.069 | 0.521 |
Rmeas | 0.074 | 0.572 |
Number of reflections | 126484 | 3941 |
<I/σ(I)> | 13.6 | 2 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 13 | |
CC(1/2) | 0.999 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293.15 | 16%(w/v) PEG 8000, 0.1M Tris/H2SO4, pH 8.3, 0.09 M K2SO4, 2mM 8-azaxanthine |