7CU8
Crystal structure of the soluble domain of TiME protein from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 198.002, 198.002, 364.108 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.780 - 3.300 |
R-factor | 0.2231 |
Rwork | 0.223 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cu9 |
RMSD bond length | 0.002 |
RMSD bond angle | 1.182 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.142 | 0.142 |
Number of reflections | 64048 | 6276 |
<I/σ(I)> | 72.3 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 21.7 | 22.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 289.15 | 0.2M lithium sulfate monohydrate, 0.1M Tris pH 8.8, 15% PEG 3350 |