7CTX

Crystal structure of Arabidopsis thaliana SOBIR1 kinase domain(residues 388-401 deleted) in complex with AMP-PNP and magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	72.389, 50.732, 93.066
Unit cell angles	90.00, 106.65, 90.00

Refinement procedure

Resolution	28.629 - 2.907
R-factor	0.2259
Rwork	0.225
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ctv
RMSD bond length	0.003
RMSD bond angle	0.541
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.097	0.465
Rmeas	0.107	0.525
Rpim	0.046	0.236
Number of reflections	14273	1371
<I/σ(I)>	14.6	2.2
Completeness [%]	98.1	95.5
Redundancy	5	4.4
CC(1/2)	0.999	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1 M Hepes (pH 7.0), 20% PEG8000