7CTX
Crystal structure of Arabidopsis thaliana SOBIR1 kinase domain(residues 388-401 deleted) in complex with AMP-PNP and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.389, 50.732, 93.066 |
| Unit cell angles | 90.00, 106.65, 90.00 |
Refinement procedure
| Resolution | 28.629 - 2.907 |
| R-factor | 0.2259 |
| Rwork | 0.225 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ctv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.541 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.097 | 0.465 |
| Rmeas | 0.107 | 0.525 |
| Rpim | 0.046 | 0.236 |
| Number of reflections | 14273 | 1371 |
| <I/σ(I)> | 14.6 | 2.2 |
| Completeness [%] | 98.1 | 95.5 |
| Redundancy | 5 | 4.4 |
| CC(1/2) | 0.999 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Hepes (pH 7.0), 20% PEG8000 |
