7CTP

Crystal Structure of Human FAM129B/MINERVA/NIBAN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-02
Detector	BRUKER SMART 6500
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	55.559, 56.283, 201.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.110 - 1.800
Rwork	0.204
R-free	0.23550
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.110	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	59778	5794
<I/σ(I)>	11.7
Completeness [%]	99.9
Redundancy	7.5
CC(1/2)	0.998	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2 M lithium acetate, 20% (w/v) PEG 3350