7CTN

Structure of the 328-692 fragment of FlhA (E351A/D356A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	71.710, 96.150, 114.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.520 - 2.800
R-factor	0.2374
Rwork	0.232
R-free	0.29040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ai0
RMSD bond length	0.003
RMSD bond angle	0.680
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.520	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.074	0.317
Number of reflections	19358	2698
<I/σ(I)>	8.1	2.8
Completeness [%]	97.1
Redundancy	3.4
CC(1/2)	0.995	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tirs HCl pH 8.5, 20 % (w/v) PEG 8000, 0.2M MgCl2