7CT3
Crystal Structure of MglC from Myxococcus xanthus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97800 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 96.690, 96.690, 58.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.540 - 1.850 |
| R-factor | 0.1604 |
| Rwork | 0.158 |
| R-free | 0.21530 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.253 |
| Data reduction software | iMOSFLM (7.2.2) |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | AutoSol (1.12) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.140 | 29.140 | 1.890 |
| High resolution limit [Å] | 1.850 | 9.060 | 1.850 |
| Rmerge | 0.086 | 0.033 | 1.455 |
| Rmeas | 0.089 | 0.035 | 1.500 |
| Rpim | 0.022 | 0.010 | 0.360 |
| Total number of observations | 226964 | 1687 | 14252 |
| Number of reflections | 14178 | 156 | 845 |
| <I/σ(I)> | 21.3 | 49.6 | 2.6 |
| Completeness [%] | 99.9 | 95.7 | 99.6 |
| Redundancy | 16 | 10.8 | 16.9 |
| CC(1/2) | 0.999 | 1.000 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.2 M Lithium Sulfate Monohydrate, 0.1 M Tris-HCl pH 8.5, 30% w/v PEG 4000 |
