7CRX
Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif 100 ps after light activation (2.63mJ/mm2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2018-10-23 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.3 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.370, 50.090, 69.390 |
Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
Resolution | 20.640 - 1.850 |
R-factor | 0.2611 |
Rwork | 0.259 |
R-free | 0.30760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g28 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.251 |
Data reduction software | CrystFEL (0.8) |
Data scaling software | CrystFEL (0.8) |
Phasing software | PHENIX (1.18.2) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.900 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 33081 | 1709 |
<I/σ(I)> | 5.17 | 1.54 |
Completeness [%] | 100.0 | 100 |
Redundancy | 268 | 63.2 |
CC(1/2) | 0.979 | 0.692 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | monoolein mixture in syringe |