7CRT
Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif 100 ps after light activation (0.17mJ/mm2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-10-23 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.3 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.370, 50.090, 69.390 |
| Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
| Resolution | 20.640 - 1.850 |
| R-factor | 0.3591 |
| Rwork | 0.357 |
| R-free | 0.40610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g28 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.679 |
| Data reduction software | CrystFEL (0.8) |
| Data scaling software | CrystFEL (0.8) |
| Phasing software | PHENIX (1.18.2) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.900 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 34776 | 1694 |
| <I/σ(I)> | 6.65 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 596.9 | 150.5 |
| CC(1/2) | 0.988 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | mix with monoolein in syringe |
