7CRM

Aminoglycoside 2'-N-acetyltransferase from Mycolicibacterium smegmatis-APO Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	69.008, 81.212, 129.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.421 - 2.487
R-factor	0.1968
Rwork	0.193
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m44
RMSD bond length	0.007
RMSD bond angle	0.948
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.487	2.500
Rmerge	0.113	0.475
Number of reflections	26178	1297
<I/σ(I)>	31.7
Completeness [%]	99.8	100
Redundancy	6.6	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M ammonium acetate, 0.1M Bis-Tris (pH 6.5), 20% (w/v) PEG 3350