7CRI
1 ps Structure of Chloride ion pumping rhodopsin (ClR) with NTQ motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2018-10-21 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.3 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.370, 50.090, 69.390 |
Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
Resolution | 20.640 - 1.850 |
R-factor | 0.299 |
Rwork | 0.295 |
R-free | 0.37250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g28 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.470 |
Data reduction software | CrystFEL (0.8) |
Data scaling software | CrystFEL (0.8) |
Phasing software | PHENIX (1.16_3549) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.900 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Number of reflections | 35361 | 1736 |
<I/σ(I)> | 5.17 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 306 | 67 |
CC(1/2) | 0.977 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | monoolein (1-oleoyl-rac-glycerol) LCP |