7CRG
Beta-strand-mediated dimeric formation of the extended Ig-like domains of human lamin A/C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2018-10-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 92.648, 92.648, 205.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.380 - 1.800 |
R-factor | 0.1942 |
Rwork | 0.193 |
R-free | 0.21560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ifr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.823 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rpim | 0.073 | |
Number of reflections | 47954 | 47954 |
<I/σ(I)> | 34.2 | |
Completeness [%] | 98.1 | |
Redundancy | 24.5 | |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.2 M Potassium Chloride, 0.05 M HEPES pH 7.5, 35%(v/v) Pentaerythritol Propoxylate (5/4PO/OH) |