7CRE
hnRNPK KH3 domain in complex with a ssDNA fragment from the SIRLOIN element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-05-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 61.611, 61.611, 144.544 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.26 |
Rwork | 0.258 |
R-free | 0.27920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zzi |
RMSD bond length | 0.004 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.151 | 0.163 | |
Rmeas | 0.159 | 0.172 | |
Rpim | 0.049 | 0.054 | 0.889 |
Total number of observations | 42330 | ||
Number of reflections | 3670 | 424 | 356 |
<I/σ(I)> | 13.7 | ||
Completeness [%] | 98.8 | 94.9 | 100 |
Redundancy | 11.5 | 9.9 | 11.1 |
CC(1/2) | 0.979 | 0.324 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25 % w/v Polyethylene glycol 3350, 100 mM BIS-TRIS pH 5.5, 200 mM Ammonium acetate |