7CQV

Complex of TRP_CBS1 and Calmodulin_Nlobe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97890
Spacegroup name	H 3 2
Unit cell lengths	98.449, 98.449, 134.263
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.220 - 1.780
R-factor	0.2063
Rwork	0.205
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1up5
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.780	4.830	1.780
Rmerge	0.061	0.063	0.356
Rmeas	0.063	0.065	0.365
Rpim	0.014	0.015	0.082
Total number of observations	473415
Number of reflections	24105	1288	1192
<I/σ(I)>	8.6
Completeness [%]	100.0	99.9	100
Redundancy	19.6	19.1	19.5
CC(1/2)		0.994	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	2.5M Ammonium sulfate, 0.1M BIS-TRIS propane pH 7.0