7CQQ
GmaS in complex with AMPPNP and MetSox
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 115.553, 174.476, 190.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.826 - 2.295 |
R-factor | 0.1591 |
Rwork | 0.157 |
R-free | 0.19550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cql |
RMSD bond length | 0.007 |
RMSD bond angle | 1.121 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.295 | 6.240 | 2.300 |
Rmerge | 0.085 | 0.037 | 0.449 |
Rmeas | 0.092 | 0.040 | 0.492 |
Rpim | 0.036 | 0.016 | 0.199 |
Number of reflections | 85608 | 4527 | 4214 |
<I/σ(I)> | 5.9 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 6.4 | 6.4 | 6 |
CC(1/2) | 0.999 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M bicine (pH 8.5), 10% (v/v) 2-propanol, 30% (v/v) polyethylene glycol 1500 |