7CQO

Lysozyme grown in LCP soaked with selenourea for 6 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	78.733, 78.733, 37.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.660 - 1.710
R-factor	0.1704
Rwork	0.169
R-free	0.19880
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.616
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.770
High resolution limit [Å]	1.710	1.710
Rmerge	0.072	0.487
Rpim	0.014	0.097
Number of reflections	13134	1278
<I/σ(I)>	51.3	8.08
Completeness [%]	99.5	97.1
Redundancy	25.5	22.9
CC(1/2)	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.5	297	35% (v/v) PEG 400, 0.8 M NaCl, 100 mM sodium citrate/citric acid pH 4.5