7CQM
PlsY grown in LCP soaked with selenourea for 22 min
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.877, 88.990, 53.982 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 36.370 - 1.800 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19020 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.094 | 0.973 |
| Rpim | 0.039 | 0.400 |
| Number of reflections | 46884 | 4730 |
| <I/σ(I)> | 1.69 | 18.59 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 1.000 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 25-35% (v/v) triethylene glycol, 0.1 M ammonium sulfate, 0.1 M glycine |
