7CQL

Apo GmaS without ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	115.909, 179.024, 192.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.573 - 2.801
R-factor	0.203
Rwork	0.200
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lni
RMSD bond length	0.009
RMSD bond angle	1.216
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.034	2.800
Rmerge	0.128	0.073	0.310
Rmeas	0.134	0.077	0.325
Rpim	0.040	0.022	0.099
Total number of observations	545098
Number of reflections	48331	5064	4782
<I/σ(I)>	15.4
Completeness [%]	98.6	98.8	99.1
Redundancy	11.3	12.1	10.7
CC(1/2)		0.998	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES (pH 6.5), 1 M lithium sulfate