7CPO

Crystal Structure of Anolis carolinensis MHC I complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978
Spacegroup name	I 2 2 2
Unit cell lengths	40.919, 93.690, 246.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.500
R-factor	0.1884
Rwork	0.185
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjx
RMSD bond length	0.009
RMSD bond angle	1.115
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.390
High resolution limit [Å]	1.400	2.090
Rmerge	0.143	0.381
Rmeas	0.150	0.396
Rpim	0.044	0.107
Number of reflections	72758	9478
<I/σ(I)>	7.7	6.5
Completeness [%]	77.0	100
Redundancy	10.5
CC(1/2)		0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.2 M Potassium chloride, 0.1M Magnesium acetate tetrahydrate, 0.05M Sodium cacodylate trihydrate (pH 6.5), 10% w/v PEG 8000