7CPH

Crystal structure of tRNA adenosine deaminase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	38.530, 41.250, 202.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.190 - 2.300
R-factor	0.1811
Rwork	0.177
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3j
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.520	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.126	0.514
Rmeas	0.142	0.574
Rpim	0.064	0.248
Number of reflections	13799	2067
<I/σ(I)>	7.9
Completeness [%]	91.5	95.4
Redundancy	4.4	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	8% tacsimate pH 6.0, 20% PEG3350