7CPC
His-Mediated Reversible Self-assembly of Ferritin Nanocage with Ni binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97892 |
Spacegroup name | I 4 |
Unit cell lengths | 125.845, 125.845, 176.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.275 - 2.801 |
R-factor | 0.199 |
Rwork | 0.195 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kh1 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 33438 | 1668 |
<I/σ(I)> | 7.545 | |
Completeness [%] | 99.3 | |
Redundancy | 4.6 | |
CC(1/2) | 0.959 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | 1.26 M (NH4)2SO4, 100 mM Tris (pH =8.0) |