7CP5
Bovine heart cytochrome c oxidase in a catalytic intermediate of E at 1.76 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 50 |
| Detector technology | CCD |
| Collection date | 2014-05-27 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 181.589, 203.218, 177.702 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.905 - 1.760 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.18860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b1a |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.587 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.000 | 1.780 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.102 | 0.915 |
| Rpim | 0.029 | |
| Number of reflections | 644860 | 15945 |
| <I/σ(I)> | 37.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.7 | 277 | PEG 4000, SODIUM PHOSPHATE BUFFER |
