7COE
Crystal structure of Receptor binding domain of MERS-CoV and KNIH90-F1 Fab complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.590, 146.819, 81.622 |
Unit cell angles | 90.00, 96.73, 90.00 |
Refinement procedure
Resolution | 44.950 - 2.050 |
Rwork | 0.176 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zs6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.890 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.950 | 2.123 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 103522 | 9762 |
<I/σ(I)> | 14.97 | |
Completeness [%] | 99.0 | |
Redundancy | 7 | |
CC(1/2) | 0.956 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | 0.1M MES monohydrate pH 6.3, 15%(w/v) PEG 20000 |