7CO8

Ternary complex of DNA polymerase Mu with 2-nt gapped DNA (T:dGMPNPP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	95
Detector technology	CCD
Collection date	2019-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	60.310, 69.200, 117.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.084 - 1.697
R-factor	0.1799
Rwork	0.179
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.084	29.084	1.740
High resolution limit [Å]	1.697	7.590	1.700
Rmerge	0.077	0.055	0.529
Rmeas	0.085	0.062	0.601
Number of reflections	54867	679	3809
<I/σ(I)>	13.44	26.17	2.89
Completeness [%]	99.2	95.9	94.8
Redundancy	5.357	4.583	4.376
CC(1/2)	0.997	0.994	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	KCL, BIS-TRIS, PEG 10000