7CNZ
Crystal structure of 10PE bound PSD from E. coli (2.70 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.723, 102.373, 168.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.430 - 2.700 |
| R-factor | 0.2295 |
| Rwork | 0.227 |
| R-free | 0.27630 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.514 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.470 | 29.470 | 2.820 |
| High resolution limit [Å] | 2.700 | 9.350 | 2.700 |
| Rmerge | 0.185 | 0.029 | 2.649 |
| Rmeas | 0.192 | 0.030 | 2.757 |
| Rpim | 0.052 | 0.009 | 0.757 |
| Total number of observations | 529506 | 12610 | 61221 |
| Number of reflections | 38760 | 1015 | 4666 |
| <I/σ(I)> | 12.6 | 63.1 | 1 |
| Completeness [%] | 99.9 | 96.8 | 100 |
| Redundancy | 13.7 | 12.4 | 13.1 |
| CC(1/2) | 0.999 | 0.999 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5, 25% w/v PEG 3350 |
