7CNC

cystal structure of human ERH in complex with DGCR8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	86.848, 45.072, 43.479
Unit cell angles	90.00, 107.44, 90.00

Refinement procedure

Resolution	39.593 - 1.600
R-factor	0.169
Rwork	0.168
R-free	0.18590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w9g
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.086	0.052	0.406
Rmeas	0.093	0.056	0.454
Rpim	0.036	0.021	0.197
Total number of observations	127866
Number of reflections	20184	1100	751
<I/σ(I)>	5.5
Completeness [%]	94.8	99	71.8
Redundancy	6.3	6.6	4.5
CC(1/2)		0.997	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Imidazole malate pH 6, 8% w/v PEG4000