7CN6

T4 phage spackle protein gp61.3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	30.376, 46.921, 75.277
Unit cell angles	90.00, 93.08, 90.00

Refinement procedure

Resolution	19.900 - 1.600
R-factor	0.1814
Rwork	0.179
R-free	0.22790
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.800	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.107	0.190
Rmeas	0.151	0.269
Rpim	0.107	0.190
Number of reflections	51366	1001
<I/σ(I)>	4	2.1
Completeness [%]	95.2	75.4
Redundancy	1.9
CC(1/2)	0.979	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.4	293.15	PEG4000, glycine, calcium chloride