7CMR

The Crystal Structure of human MYST1 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	46.399, 58.322, 121.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.476 - 2.200
Rwork	0.218
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2giv
RMSD bond length	0.002
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.076	0.282
Number of reflections	16888	833
<I/σ(I)>	20.8
Completeness [%]	96.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M LiCl, 0.1M Hepes pH7, 20% PEG6,000