7CMQ
Crystal Structure of Bacillus sp. TB-90 Urate Oxidase Improved by Humidity Control at 88% RH.
Replaces: 5Z2AExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 71.063, 133.443, 144.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.100 - 1.650 |
R-factor | 0.1701 |
Rwork | 0.169 |
R-free | 0.19740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wlv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.036 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.100 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.054 | 0.673 |
Rmeas | 0.063 | 0.788 |
Number of reflections | 82336 | 25541 |
<I/σ(I)> | 13.96 | 2.12 |
Completeness [%] | 99.4 | 98.8 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.998 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% PEG 8000, 0.1 M Tris-HCl, 0.08 M K2SO4, 2 mM 8-azaxthantine |