7CMP
parE in complex with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.016, 126.747, 96.044 |
Unit cell angles | 90.00, 109.61, 90.00 |
Refinement procedure
Resolution | 46.104 - 2.886 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.25990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lps |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.940 |
High resolution limit [Å] | 2.886 | 2.890 |
Number of reflections | 37042 | 1849 |
<I/σ(I)> | 21.2 | |
Completeness [%] | 98.5 | |
Redundancy | 3.9 | |
CC(1/2) | 0.980 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 45% v/v 2-metyl-2,4-pentanediol |