7CMN
Crystal Structure of Bacillus sp. TB-90 Urate Oxidase Improved by Humidity Control at 88% RH.
Replaces: 5Z29Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 70.889, 132.912, 144.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.230 - 1.420 |
R-factor | 0.2011 |
Rwork | 0.200 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wlv |
RMSD bond length | 0.009 |
RMSD bond angle | 0.878 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.230 | 1.500 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.061 | 0.427 |
Rmeas | 0.072 | 0.502 |
Number of reflections | 127636 | 39778 |
<I/σ(I)> | 11.67 | 2.7 |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.997 | 0.829 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 16% PEG 8000, 0.1 M Tris-HCl, 0.08 M K2SO4, 2 mM 8-azaxthantine |