7CML
The Crystal Structure of human JNK2 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 4 |
| Unit cell lengths | 102.004, 102.004, 70.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.616 - 2.150 |
| Rwork | 0.284 |
| R-free | 0.32740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3npc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.616 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.141 | 1.269 |
| Number of reflections | 39267 | 3435 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Na/K PO4, 20% PEG3,350 |
